Genome architectures revealed by tethered chromosome conformation capture and population-based modeling.

ABSTRACT: We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes. We identified a group of regions in each chromosome in human cells that account for the majority of interchromosomal interactions. These regions are marked by high transcriptional activity, suggesting that their interactions are mediated by transcriptional machinery. Each of these regions interacts with numerous other such regions throughout the genome in an indiscriminate fashion, partly driven by the accessibility of the partners. As a different combination of interactions

SUBMITTER: Kalhor R 

PROVIDER: S-EPMC3782096 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature