Neuroprotective effects of microRNA-210 on hypoxic-ischemic encephalopathy.

ABSTRACT:

Objectives

To reveal the effect of microRNA-210 on cell apoptosis caused by HIE.

Methods

Postnatal day 7 rats after HI injury were intraventricularly injected with microRNA-210 mimic, microRNA-210 inhibitor, or physiological saline. 72 h after the injection, rats were sacrificed and the left hemispheres were collected. The expression level of microRNA-210 was identified by quantitative real-time PCR analysis. Apoptosis in brain sections was investigated by TUNEL assay. Apoptosis-related protein expressions were studied by Western blot analysis.

Results

The results showed that microRNA-210, whose expression was downregulated in the brain 72 h after HI injury, suppressed neuronal apoptosis by inhibiting caspase activity and regulating the balance between bcl-2 and bax l

SUBMITTER: Qiu J 

PROVIDER: S-EPMC3782121 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature