HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium.

ABSTRACT:

Background

While acute lung injury (ALI) contributes significantly to critical illness, it resolves spontaneously in many instances. The majority of patients experiencing ALI require mechanical ventilation. Therefore, we hypothesized that mechanical ventilation and concomitant stretch-exposure of pulmonary epithelia could activate endogenous pathways important in lung protection.

Methods and findings

To examine transcriptional responses during ALI, we exposed pulmonary epithelia to cyclic mechanical stretch conditions--an in vitro model resembling mechanical ventilation. A genome-wide screen revealed a transcriptional response similar to hypoxia signaling. Surprisingly, we found that stabilization of hypoxia-inducible factor 1A (HIF1A) during stretch conditions in vitro or d

SUBMITTER: Eckle T 

PROVIDER: S-EPMC3782424 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature