Efficient cytokine-induced IL-13 production by mast cells requires both IL-33 and IL-3.

ABSTRACT:

Background

IL-13 is a critical effector cytokine for allergic inflammation. It is produced by several cell types, including mast cells, basophils, and TH2 cells. In mast cells and basophils its induction can be stimulated by cross-linkage of immunoglobulin receptors or cytokines. The IL-1 family members IL-33 and IL-18 have been linked to induction of IL-13 production by mast cells and basophils. In CD4 TH2 cells IL-33-mediated production of IL-13 requires simultaneous signal transducer and activator of transcription (STAT) 5 activation.

Objective

Here we have addressed whether cytokine-induced IL-13 production in mast cells and basophils follows the same logic as in TH2 cells: requirement of 2 separate signals.

Methods

By generating a bacterial artificial chromosome

SUBMITTER: Junttila IS 

PROVIDER: S-EPMC3782850 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature