Site-specific incorporation of ε-N-crotonyllysine into histones.

ABSTRACT: A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.

SUBMITTER: Kim CH 

PROVIDER: S-EPMC3783207 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature