Rhizobium pongamiae sp. nov. from root nodules of Pongamia pinnata.

ABSTRACT: Pongamia pinnata has an added advantage of N2-fixing ability and tolerance to stress conditions as compared with other biodiesel crops. It harbours "rhizobia" as an endophytic bacterial community on its root nodules. A gram-negative, nonmotile, fast-growing, rod-shaped, bacterial strain VKLR-01(T) was isolated from root nodules of Pongamia that grew optimal at 28°C, pH 7.0 in presence of 2% NaCl. Isolate VKLR-01 exhibits higher tolerance to the prevailing adverse conditions, for example, salt stress, elevated temperatures and alkalinity. Strain VKLR-01(T) has the major cellular fatty acid as C(18:1) ω7c (65.92%). Strain VKLR-01(T) was found to be a nitrogen fixer using the acetylene reduction assay and PCR detection of a nifH gene. On the basis of phenotypic, phylogenetic distinctiveness a

SUBMITTER: Kesari V 

PROVIDER: S-EPMC3783817 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature