HERV-K and LINE-1 DNA Methylation and Reexpression in Urothelial Carcinoma.

ABSTRACT: Changes in DNA methylation frequently accompany cancer development. One prominent change is an apparently genome-wide decrease in methylcytosine that is often ascribed to DNA hypomethylation at retroelements comprising nearly half the genome. DNA hypomethylation may allow reactivation of retroelements, enabling retrotransposition, and causing gene expression disturbances favoring tumor development. However, neither the extent of hypomethylation nor of retroelement reactivation are precisely known. We therefore assessed DNA methylation and expression of three major classes of retroelements (LINE-1, HERV-K, and AluY) in human urinary bladder cancer tissues and cell lines by pyrosequencing and quantitative reverse transcription-polymerase chain reaction, respectively. We found substantial glo

SUBMITTER: Kreimer U 

PROVIDER: S-EPMC3783855 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature