Clinical relevance of cancer stem cells in bone marrow of early breast cancer patients.

ABSTRACT:

Background

Cancer stem cells (CSCs) are epithelial tumor cells that express CD44(+)CD24(-/lo). CSCs can be further divided into those that have aldehyde dehydrogenase (ALDH) activity (Aldefluor(+)) and those that do not. We hypothesized that if CSCs are responsible for tumor dissemination, their presence in bone marrow (BM) would be prognostic in early stages of breast cancer (EBC) patients.

Patients and methods

BM aspirates were collected at the time of surgery from 108 patients with EBC. BM was analyzed for CSCs and ALDH activity by flow cytometry. Overall survival and disease-free survival (DFS) were calculated from the date of diagnosis and analyzed with Kaplan-Meier survival plots. Cox multivariate proportional hazards model was also carried out.

Results

Patients

SUBMITTER: Giordano A 

PROVIDER: S-EPMC3784333 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature