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PARP-1 regulates expression of TGF-? receptors in T cells.

ABSTRACT: Transforming growth factor-? (TGF-?) receptors (T?Rs) are essential components for TGF-? signal transduction in T cells, yet the mechanisms by which the receptors are regulated remain poorly understood. We show here that Poly(ADP-ribose) polymerase-1 (PARP-1) regulates TGF-? receptor I (T?RI) and II (T?RII) expression in CD4(+) T cells and subsequently affects Smad2/3-mediated TGF-? signal transduction. Inhibition of PARP-1 led to the upregulation of both T?RI and T?RII, yet the underlying molecular mechanisms were distinct. PARP-1 selectively bound to the promoter of T?RII, whereas the enzymatic activity of PARP-1 was responsible for the inhibition of T?RI expression. Importantly, inhibition of PARP-1 also enhanced expression of T?Rs in human CD4(+) T cells. Thus, PARP-1 regulates T?R expression and TGF-? signaling in T cells.

SUBMITTER: Zhang P

PROVIDER: S-EPMC3785120 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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PARP-1 regulates expression of TGF-β receptors in T cells.

Zhang Pin P Nakatsukasa Hiroko H Tu Eric E Kasagi Shimpei S Cui Kairong K Ishikawa Masaki M Konkel Joanne E JE Maruyama Takashi T Wei Gang G Abbatiello Brittany B Wang Zhao-Qi ZQ Zhao Keji K Chen WanJun W

Blood 20130812 13

Transforming growth factor-β (TGF-β) receptors (TβRs) are essential components for TGF-β signal transduction in T cells, yet the mechanisms by which the receptors are regulated remain poorly understood. We show here that Poly(ADP-ribose) polymerase-1 (PARP-1) regulates TGF-β receptor I (TβRI) and II (TβRII) expression in CD4(+) T cells and subsequently affects Smad2/3-mediated TGF-β signal transduction. Inhibition of PARP-1 led to the upregulation of both TβRI and TβRII, yet the underlying molec ...[more]

PMID: 23940283

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