Molecular Insights on Post-chemotherapy Retinoblastoma by Microarray Gene Expression Analysis.

ABSTRACT:

Purpose

Management of Retinoblastoma (RB), a pediatric ocular cancer is limited by drug-resistance and drug-dosage related side effects during chemotherapy. Molecular de-regulation in post-chemotherapy RB tumors was investigated.

Materials and methods

cDNA microarray analysis of two post-chemotherapy and one pre-chemotherapy RB tumor tissues was performed, followed by Principle Component Analysis, Gene ontology, Pathway Enrichment analysis and Biological Analysis Network (BAN) modeling. The drug modulation role of two significantly up-regulated genes (p≤0.05) - Ect2 (Epithelial-cell-transforming-sequence-2), and PRAME (preferentially-expressed-Antigen-in-Melanoma) was assessed by qRT-PCR, immunohistochemistry and cell viability assays.

Results

Differential up-regulati

SUBMITTER: Nalini V 

PROVIDER: S-EPMC3785389 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature