Circulating liver-specific miR-122 as a novel potential biomarker for diagnosis of cholestatic liver injury.

ABSTRACT:

Background

Circulating microRNA-122 (miR-122) has been increasingly reported to be a potential biomarker for drug-, viral-, alcohol- and chemical-induced liver injury. The present study was initiated to determine the potential of circulating miR-122 as a biomarker for cholestatic liver injury.

Methods

Both bile-duct ligation (BDL) mice and patients with biliary calculi were employed as cholestatic liver injury models, and serum miR-122 level was determined by stem-loop real-time reverse-transcription PCR (SLqRT-PCR). All quantitative PCR values were normalized to those for U6 RNA and calculated with the 2(-△Ct) method.

Results

Serum miR-122 increased significantly after BDL-induced cholestatic injury and showed a similar time course to ALT concentrations. Compared wit

SUBMITTER: Shifeng H 

PROVIDER: S-EPMC3785475 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature