Semisynthetic K+ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state.

ABSTRACT: C-type inactivation of K(+) channels plays a key role in modulating cellular excitability. During C-type inactivation, the selectivity filter of a K(+) channel changes conformation from a conductive to a nonconductive state. Crystal structures of the KcsA channel determined at low K(+) or in the open state revealed a constricted conformation of the selectivity filter, which was proposed to represent the C-type inactivated state. However, structural studies on other K(+) channels do not support the constricted conformation as the C-type inactivated state. In this study, we address whether the constricted conformation of the selectivity filter is in fact the C-type inactivated state. The constricted conformation can be blocked by substituting the first conserved glycine in the selectivity fi

SUBMITTER: Devaraneni PK 

PROVIDER: S-EPMC3785774 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature