Using antiubiquitin antibodies to probe the ubiquitination state within rhTRIM5α cytoplasmic bodies.

ABSTRACT: The first line of defense protecting rhesus macaques from HIV-1 is the restriction factor rhTRIM5α, which recognizes the capsid core of the virus early after entry and normally blocks infection prior to reverse transcription. Cytoplasmic bodies containing rhTRIM5α have been implicated in the ubiquitin-proteasome pathway, but the specific roles these structures play remain uncharacterized. Here, we examine the ubiquitination status of cytoplasmic body proteins. Using antibodies specific for different forms of ubiquitin, we show that ubiquitinated proteins are present in cytoplasmic bodies, and that this localization is altered after proteasome inhibition. A decrease in polyubiquitinated proteins localizing to cytoplasmic bodies was apparent after 1 h of proteasome inhibition, and greater di

SUBMITTER: Danielson CM 

PROVIDER: S-EPMC3785812 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature