Urinary-cell mRNA profile and acute cellular rejection in kidney allografts.

ABSTRACT:

Background

The standard test for the diagnosis of acute rejection in kidney transplants is the renal biopsy. Noninvasive tests would be preferable.

Methods

We prospectively collected 4300 urine specimens from 485 kidney-graft recipients from day 3 through month 12 after transplantation. Messenger RNA (mRNA) levels were measured in urinary cells and correlated with allograft-rejection status with the use of logistic regression.

Results

A three-gene signature of 18S ribosomal (rRNA)-normalized measures of CD3ε mRNA and interferon-inducible protein 10 (IP-10) mRNA, and 18S rRNA discriminated between biopsy specimens showing acute cellular rejection and those not showing rejection (area under the curve [AUC], 0.85; 95% confidence interval [CI], 0.78 to 0.91; P<0.001 by re

SUBMITTER: Suthanthiran M 

PROVIDER: S-EPMC3786188 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature