Ontology highlight
ABSTRACT:
SUBMITTER: Nichols RC
PROVIDER: S-EPMC3786348 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Molecular biotechnology 20110701 3
The study of post-transcriptional regulation is constrained by the technical limitations associated with both transient and stable transfection of chimeric reporter plasmids examining the activity of 3'-UTR cis-acting elements. We report the adaptation of a commercially available system that enables consistent stable integration of chimeric reporter cDNA into a single genomic site in which transcription is induced by tetracycline. Using this system, we demonstrate the tight control afforded by t ...[more]