Autoimmunoreactive IgGs from patients with postural orthostatic tachycardia syndrome.

ABSTRACT:

Purpose

Autoantibodies are implicated in the pathogenesis of cardiovascular diseases and cardiac arrhythmias. In this pilot study, we tested the hypothesis that autoantibodies are present in patients with postural orthostatic tachycardia syndrome (POTS).

Experimental design

Seven control subjects (6 F:1 M, average age 36.1 years) and ten patients with the diagnosis of POTS (7 F: 3 M, average age 35.1 years) provided informed consent and 30 mL of venous blood. Human heart membrane proteins were resolved by 2DE and immunoblotted against purified IgGs from controls and patients.

Results

Eighteen protein spots immunoreactive specifically against patient IgGs were detected and they were excised from gels, trypsin-digested, and analyzed by nanoLC-electrospray MS/MS. Forty u

SUBMITTER: Wang XL 

PROVIDER: S-EPMC3786406 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature