Solubilization and stabilization of isolated photosystem I complex with lipopeptide detergents.

ABSTRACT: It is difficult to maintain a target membrane protein in a soluble and functional form in aqueous solution without biological membranes. Use of surfactants can improve solubility, but it remains challenging to identify adequate surfactants that can improve solubility without damaging their native structures and biological functions. Here we report the use of a new class of lipopeptides to solubilize photosystem I (PS-I), a well known membrane protein complex. Changes in the molecular structure of these surfactants affected their amphiphilicity and the goal of this work was to exploit a delicate balance between detergency and biomimetic performance in PS-I solubilization via their binding capacity. Meanwhile, the effects of these surfactants on the thermal and structural stability and funct

SUBMITTER: Wang X 

PROVIDER: S-EPMC3787008 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature