Adrenomedullin is up-regulated in patients with pancreatic cancer and causes insulin resistance in β cells and mice.

ABSTRACT:

Background & aims

New-onset diabetes in patients with pancreatic cancer is likely to be a paraneoplastic phenomenon caused by tumor-secreted products. We aimed to identify the diabetogenic secretory product(s) of pancreatic cancer.

Methods

Using microarray analysis, we identified adrenomedullin as a potential mediator of diabetes in patients with pancreatic cancer. Adrenomedullin was up-regulated in pancreatic cancer cell lines, in which supernatants reduced insulin signaling in beta cell lines. We performed quantitative reverse-transcriptase polymerase chain reaction and immunohistochemistry on human pancreatic cancer and healthy pancreatic tissues (controls) to determine expression of adrenomedullin messenger RNA and protein, respectively. We studied the effects of adrenom

SUBMITTER: Aggarwal G 

PROVIDER: S-EPMC3787599 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature