Gene expression profile in human trabecular meshwork from patients with primary open-angle glaucoma.

ABSTRACT:

Purpose

To identify the specific genes in human trabecular meshwork (TM) related to POAG.

Methods

Primary open-angle glaucoma TM specimens were obtained from routine trabeculectomy surgery. Nonglaucomatous control TM specimens were dissected from donor eyes using the same approach as a standard trabeculectomy. All cases were screened for myocilin (MYOC) mutations. Total RNA was extracted, labeled, and hybridized to Illumina HumanWG-6 BeadChips. Expression data were normalized and analyzed using the R package limma in Bioconductor. Pathway analyses were performed using DAVID Bioinformatics Resources.

Results

Our study included surgical TM specimens from 15 cases and 13 controls. One case was identified with a heterozygous Q368X MYOC mutation. If TMs were available from

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3787658 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature