Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.

ABSTRACT: Lipid droplets (LD) are the main depot of non-polar lipids in all eukaryotic cells. In the present study we describe isolation and characterization of LD from the industrial yeast Pichia pastoris. We designed and adapted an isolation procedure which allowed us to obtain this subcellular fraction at high purity as judged by quality control using appropriate marker proteins. Components of P. pastoris LD were characterized by conventional biochemical methods of lipid and protein analysis, but also by a lipidome and proteome approach. Our results show several distinct features of LD from P. pastoris especially in comparison to Saccharomyces cerevisiae. P. pastoris LD are characterized by their high preponderance of triacylglycerols over steryl esters in the core of the organelle, the high degr

SUBMITTER: Ivashov VA 

PROVIDER: S-EPMC3787741 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature