CD3γ/δ in sea bass (Dicentrarchus labrax): Molecular characterization and expression analysis.

ABSTRACT: The CD3 complex is the common marker on the surface of both αβ and γδ T cells and is essential for formation of the T-cell receptor complex and for T-cell activation. In this paper, we report the gene cloning and molecular characterization of a CD3γ/δ homologue in sea bass (Dicentrarchus labrax), the analysis of transcription levels in lymphoid and non-lymphoid organs and the gene regulation after in vitro stimulation with LPS and PHA. Four cysteine residues in the extracellular domain, involved in the constitution of immunoglobulin-like domain, are present in sea bass CD3γ/δ sequence and they are conserved both in number and position from mammals to teleost sequences. Similar to other known CD3γ/δs, in sea bass CD3γ/δ there is also a conserved immunoreceptor tyrosine-based activation ITAM

SUBMITTER: Randelli E 

PROVIDER: S-EPMC3787811 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature