Role of focal adhesion kinase in regulating YB-1-mediated paclitaxel resistance in ovarian cancer.

ABSTRACT:

Background

We previously found focal adhesion kinase (FAK) inhibition sensitizes ovarian cancer to taxanes; however, the mechanisms are not well understood.

Methods

We characterized the biologic response of taxane-resistant and taxane-sensitive ovarian cancer models to a novel FAK inhibitor (VS-6063). We used reverse-phase protein arrays (RPPA) to identify novel downstream targets in taxane-resistant cell lines. Furthermore, we correlated clinical and pathological data with nuclear and cytoplasmic expression of FAK and YB-1 in 105 ovarian cancer samples. Statistical tests were two-sided, and P values were calculated with Student t test or Fisher exact test.

Results

We found that VS-6063 inhibited FAK phosphorylation at the Tyr397 site in a time- and dose-dependent man

SUBMITTER: Kang Y 

PROVIDER: S-EPMC3787907 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature