Weak operator binding enhances simulated Lac repressor-mediated DNA looping.

ABSTRACT: The 50th anniversary of Biopolymers coincides closely with the like celebration of the discovery of the Escherichia coli (lac) lactose operon, a classic genetic system long used to illustrate the influence of biomolecular structure on function. The looping of DNA induced by the binding of the Lac repressor protein to sequentially distant operator sites on DNA continues to serve as a paradigm for understanding long-range genomic communication. Advances in analyses of DNA structures and in incorporation of proteins in computer simulations of DNA looping allow us to address long-standing questions about the role of protein-mediated DNA loop formation in transcriptional control. Here we report insights gained from studies of the sequence-dependent contributions of the natural lac operators to

SUBMITTER: Colasanti AV 

PROVIDER: S-EPMC3788042 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature