Eosinophil peroxidase in sputum represents a unique biomarker of airway eosinophilia.

ABSTRACT:

Background

Sputum eosinophilia has been shown to be a predictor of response to anti-eosinophil therapies in patients with airway diseases. However, quantitative cell counts and differentials of sputum are labor intensive. The objective of this study was to validate a novel ELISA-based assay of eosinophil peroxidase (EPX) in sputum as a rapid and reliable marker of airway eosinophils.

Methods

The utility of EPX-based ELISA as an eosinophil-specific assay was achieved through comparisons with sputum eosinophil differential counts in freshly prepared and archived patient samples from a variety of clinical settings.

Results

EPX levels in sputum correlated with eosinophil percentage (r(s) = 0.84) in asthma patients with varying degrees of airway eosinophilia. Significantly

SUBMITTER: Nair P 

PROVIDER: S-EPMC3788081 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature