Temporal changes of Japanese encephalitits virus in different brain regions of rat.

ABSTRACT:

Background & objectives

Japanese encephalitis virus (JEV) infection results in acute encephalitic illness. The affinity of JEV to different regions of brain and temporal changes in viral load have not been studied. This study was conducted to describe localization of JEV to different regions of the brain at different stages of disease in a rat model of Japanese encephalitis (JE).

Methods

Twelve days old Wistar rats were inoculated intracerebrally with a dose of 3 x 10⁶ pfu/ml of JEV. After 3, 6, 10 and 20 days post-inoculation, brains were dissected out and different regions of brain (cortex, striatum, thalamus and mid brain) were taken. Motor deficit was assessed by the rota rod and JEV RNA copies were evaluated using real-time PCR assay.

Results

There was a signific

SUBMITTER: Srivastava R 

PROVIDER: S-EPMC3788207 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature