Using high throughput sequencing to explore the biodiversity in oral bacterial communities.

ABSTRACT: High throughput sequencing of 16S ribosomal RNA gene amplicons is a cost-effective method for characterization of oral bacterial communities. However, before undertaking large-scale studies, it is necessary to understand the technique-associated limitations and intrinsic variability of the oral ecosystem. In this work we evaluated bias in species representation using an in vitro-assembled mock community of oral bacteria. We then characterized the bacterial communities in saliva and buccal mucosa of five healthy subjects to investigate the power of high throughput sequencing in revealing their diversity and biogeography patterns. Mock community analysis showed primer and DNA isolation biases and an overestimation of diversity that was reduced after eliminating singleton operational taxonomi

SUBMITTER: Diaz PI 

PROVIDER: S-EPMC3789374 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature