Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.

ABSTRACT:

Background

Rapid desensitization transiently prevents severe allergic reactions, allowing administration of life-saving therapies in previously sensitized patients. However, the mechanisms underlying successful rapid desensitization are not fully understood.

Objectives

We sought to investigate whether the mast cell (MC) is an important target of rapid desensitization in mice sensitized to exhibit IgE-dependent passive systemic anaphylaxis in vivo and to investigate the antigen specificity and underlying mechanisms of rapid desensitization in our mouse model.

Methods

C57BL/6 mice (in vivo) or primary isolated C57BL/6 mouse peritoneal mast cells (PMCs; in vitro) were passively sensitized with antigen-specific anti-2,4-dinitrophenyl IgE, anti-ovalbumin IgE, or both. MCs

SUBMITTER: Oka T 

PROVIDER: S-EPMC3789647 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature