Proteomic profiling of the hypothalamus in a mouse model of cancer-induced anorexia-cachexia.

ABSTRACT:

Background

Anorexia-cachexia is a common and severe cancer-related complication but the underlying mechanisms are largely unknown. Here, using a mouse model for tumour-induced anorexia-cachexia, we screened for proteins that are differentially expressed in the hypothalamus, the brain's metabolic control centre.

Methods

The hypothalamus of tumour-bearing mice with implanted methylcholanthrene-induced sarcoma (MCG 101) displaying anorexia and their sham-implanted pair-fed or free-fed littermates was examined using two-dimensional electrophoresis (2-DE)-based comparative proteomics. Differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry.

Results

The 2-DE data showed an increased expression of dynamin 1, hexokinase, pyruvate ca

SUBMITTER: Ihnatko R 

PROVIDER: S-EPMC3790177 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature