Proteomic identification of novel secreted antibacterial toxins of the Serratia marcescens type VI secretion system.

ABSTRACT: It has recently become apparent that the Type VI secretion system (T6SS) is a complex macromolecular machine used by many bacterial species to inject effector proteins into eukaryotic or bacterial cells, with significant implications for virulence and interbacterial competition. "Antibacterial" T6SSs, such as the one elaborated by the opportunistic human pathogen, Serratia marcescens, confer on the secreting bacterium the ability to rapidly and efficiently kill rival bacteria. Identification of secreted substrates of the T6SS is critical to understanding its role and ability to kill other cells, but only a limited number of effectors have been reported so far. Here we report the successful use of label-free quantitative mass spectrometry to identify at least eleven substrates of the S. mar

SUBMITTER: Fritsch MJ 

PROVIDER: S-EPMC3790287 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature