Identification of methyllysine peptides binding to chromobox protein homolog 6 chromodomain in the human proteome.

ABSTRACT: Methylation is one of the important post-translational modifications that play critical roles in regulating protein functions. Proteomic identification of this post-translational modification and understanding how it affects protein activity remain great challenges. We tackled this problem from the aspect of methylation mediating protein-protein interaction. Using the chromodomain of human chromobox protein homolog 6 as a model system, we developed a systematic approach that integrates structure modeling, bioinformatics analysis, and peptide microarray experiments to identify lysine residues that are methylated and recognized by the chromodomain in the human proteome. Given the important role of chromobox protein homolog 6 as a reader of histone modifications, it was interesting to find th

SUBMITTER: Li N 

PROVIDER: S-EPMC3790288 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature