Systems-level overview of host protein phosphorylation during Shigella flexneri infection revealed by phosphoproteomics.

ABSTRACT: The enteroinvasive bacterium Shigella flexneri invades the intestinal epithelium of humans. During infection, several injected effector proteins promote bacterial internalization, and interfere with multiple host cell responses. To obtain a systems-level overview of host signaling during infection, we analyzed the global dynamics of protein phosphorylation by liquid chromatography-tandem MS and identified several hundred of proteins undergoing a phosphorylation change during the first hours of infection. Functional bioinformatic analysis revealed that they were mostly related to the cytoskeleton, transcription, signal transduction, and cell cycle. Fuzzy c-means clustering identified six temporal profiles of phosphorylation and a functional module composed of ATM-phosphorylated proteins rel

SUBMITTER: Schmutz C 

PROVIDER: S-EPMC3790303 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature