NeSSM: a Next-generation Sequencing Simulator for Metagenomics.

ABSTRACT:

Background

Metagenomics can reveal the vast majority of microbes that have been missed by traditional cultivation-based methods. Due to its extremely wide range of application areas, fast metagenome sequencing simulation systems with high fidelity are in great demand to facilitate the development and comparison of metagenomics analysis tools.

Results

We present here a customizable metagenome simulation system: NeSSM (Next-generation Sequencing Simulator for Metagenomics). Combining complete genomes currently available, a community composition table, and sequencing parameters, it can simulate metagenome sequencing better than existing systems. Sequencing error models based on the explicit distribution of errors at each base and sequencing coverage bias are incorporated in the

SUBMITTER: Jia B 

PROVIDER: S-EPMC3790878 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature