A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.
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ABSTRACT:
SUBMITTER: Pinotsi D
PROVIDER: S-EPMC3790954 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.
Pinotsi Dorothea D Buell Alexander K AK Dobson Christopher M CM Kaminski Schierle Gabriele S GS Kaminski Clemens F CF
Chembiochem : a European journal of chemical biology 20130416 7
Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case. ...[more]
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