HER-2 gene amplification in human breast cancer without concurrent HER-2 over-expression.

ABSTRACT:

Background

Testing for human epidermal growth factor receptor-2 (HER-2) in breast cancer is performed by either immunohistochemistry (IHC) or in situ hybridization (ISH). The growth factor receptor-bound protein-7 (GRB7) gene is in close proximity to HER-2 on chromosome 17q11-12 and codes a signal transduction molecule shown to be an independent adverse marker in breast cancer.

Methods

HER-2 and GRB7 protein expression from 613 frozen breast tumors was determined by Western analysis. HER-2 protein results were confirmed with IHC. Commercial HER-2 FISH was performed on a subset of tumors with multi-probe FISH used to assess the extent of HER-2 gene amplification. mRNA expression was determined by Multi-plex RT-PCR.

Results

Seven tumors with GRB7 protein over-expression

SUBMITTER: Luoh SW 

PROVIDER: S-EPMC3791222 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature