High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.

ABSTRACT: DNA replication origins are poorly characterized genomic regions that are essential to recruit and position the initiation complex to start DNA synthesis. Despite the lack of specific replicator sequences, initiation of replication does not occur at random sites in the mammalian genome. This has lead to the view that DNA accessibility could be a major determinant of mammalian origins. Here, we performed a high-resolution analysis of nucleosome architecture and initiation sites along several origins of different genomic location and firing efficiencies. We found that mammalian origins are highly variable in nucleosome conformation and initiation patterns. Strikingly, initiation sites at efficient CpG island-associated origins always occur at positions of high-nucleosome occupancy. Origin re

SUBMITTER: Lombrana R 

PROVIDER: S-EPMC3791372 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature