A role for adhesion and degranulation-promoting adapter protein in collagen-induced platelet activation mediated via integrin α(2) β(1).

ABSTRACT:

Background

Collagen-induced platelet activation is a key step in the development of arterial thrombosis via its interaction with the receptors glycoprotein (GP)VI and integrin α(2) β(1) . Adhesion and degranulation-promoting adapter protein (ADAP) regulates α(IIb) β(3) in platelets and α(L) β(2) in T cells, and is phosphorylated in GPVI-deficient platelets activated by collagen.

Objectives

To determine whether ADAP plays a role in collagen-induced platelet activation and in the regulation and function of α(2) β(1).

Methods

Using ADAP(-/-) mice and synthetic collagen peptides, we investigated the role of ADAP in platelet aggregation, adhesion, spreading, thromboxane synthesis, and tyrosine phosphorylation.

Results and conclusions

Platelet aggregation and phospho

SUBMITTER: Jarvis GE 

PROVIDER: S-EPMC3791415 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature