Activation of NF-κB after chronic ethanol intake and haemorrhagic shock/resuscitation in mice.

ABSTRACT:

Background and purpose

Chronic ethanol abuse and haemorrhagic shock are major causes of global mortality and, separately, induce profound hepato- and immune-toxic effects via activation of NF-κB. Here, we assessed the effects of chronic ethanol intake upon the pathophysiological derangements after haemorrhagic shock with subsequent resuscitation (H/R), with particular attention to the contribution of NF-κB.

Experimental approach

Transgenic NF-κB(EGFP) mice, expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-κB cis-elements were fed a Lieber-DeCarli diet containing ethanol (EtOH-diet) or an isocaloric control diet for 4 weeks and were then pairwise subjected to H/R. Liver tissues and peripheral blood were sampled at 2 or 24 h afte

SUBMITTER: Maraslioglu M 

PROVIDER: S-EPMC3791990 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature