Transcriptome-mining for single-copy nuclear markers in ferns.

ABSTRACT:

Background

Molecular phylogenetic investigations have revolutionized our understanding of the evolutionary history of ferns-the second-most species-rich major group of vascular plants, and the sister clade to seed plants. The general absence of genomic resources available for this important group of plants, however, has resulted in the strong dependence of these studies on plastid data; nuclear or mitochondrial data have been rarely used. In this study, we utilize transcriptome data to design primers for nuclear markers for use in studies of fern evolutionary biology, and demonstrate the utility of these markers across the largest order of ferns, the Polypodiales.

Principal findings

We present 20 novel single-copy nuclear regions, across 10 distinct protein-coding genes: ApP

SUBMITTER: Rothfels CJ 

PROVIDER: S-EPMC3792871 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature