Transcriptome sequencing and expression analysis of terpenoid biosynthesis genes in Litsea cubeba.

ABSTRACT:

Background

Aromatic essential oils extracted from fresh fruits of Litsea cubeba (Lour.) Pers., have diverse medical and economic values. The dominant components in these essential oils are monoterpenes and sesquiterpenes. Understanding the molecular mechanisms of terpenoid biosynthesis is essential for improving the yield and quality of terpenes. However, the 40 available L. cubeba nucleotide sequences in the public databases are insufficient for studying the molecular mechanisms. Thus, high-throughput transcriptome sequencing of L. cubeba is necessary to generate large quantities of transcript sequences for the purpose of gene discovery, especially terpenoid biosynthesis related genes.

Results

Using Illumina paired-end sequencing, approximately 23.5 million high-quality rea

SUBMITTER: Han XJ 

PROVIDER: S-EPMC3793921 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature