A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis.

ABSTRACT: Extracellular matrix interactions have essential roles in normal physiology and many pathological processes. Although the importance of extracellular matrix interactions in metastasis is well documented, systematic approaches to identify their roles in distinct stages of tumorigenesis have not been described. Here we report a novel-screening platform capable of measuring phenotypic responses to combinations of extracellular matrix molecules. Using a genetic mouse model of lung adenocarcinoma, we measure the extracellular matrix-dependent adhesion of tumour-derived cells. Hierarchical clustering of the adhesion profiles differentiates metastatic cell lines from primary tumour lines. Furthermore, we uncovered that metastatic cells selectively associate with fibronectin when in combination wi

SUBMITTER: Reticker-Flynn NE 

PROVIDER: S-EPMC3794716 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature