Targeting fungal genes by diced siRNAs: a rapid tool to decipher gene function in Aspergillus nidulans.

ABSTRACT:

Background

Gene silencing triggered by chemically synthesized small interfering RNAs (siRNAs) has become a powerful tool for deciphering gene function in many eukaryotes. However, prediction and validation of a single siRNA duplex specific to a target gene is often ineffective. RNA interference (RNAi) with synthetic siRNA suffers from lower silencing efficacy, off-target effects and is cost-intensive, especially for functional genomic studies. With the explosion of fungal genomic information, there is an increasing need to analyze gene function in a rapid manner. Therefore, studies were performed in order to investigate the efficacy of gene silencing induced by RNase III-diced-siRNAs (d-siRNA) in model filamentous fungus, Aspergillus nidulans.

Methodology/principal findings

SUBMITTER: Kalleda N 

PROVIDER: S-EPMC3794931 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature