Charting the signal trajectory in a light-oxygen-voltage photoreceptor by random mutagenesis and covariance analysis.

ABSTRACT: Modular signal receptors empower organisms to process environmental stimuli into adequate physiological responses. At the molecular level, a sensor module receives signals and processes the inherent information into changes of biological activity of an effector module. To better understand the molecular bases underpinning these processes, we analyzed signal reception and processing in the dimeric light-oxygen-voltage (LOV) blue light receptor YF1 that serves as a paradigm for the widespread Per-ARNT-Sim (PAS) signal receptors. Random mutagenesis identifies numerous YF1 variants in which biological activity is retained but where light regulation is abolished or inverted. One group of variants carries mutations within the LOV photosensor that disrupt proper coupling of the flavin-nucleotide

SUBMITTER: Gleichmann T 

PROVIDER: S-EPMC3795236 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature