Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic.

ABSTRACT: Hydrogenases catalyze the formation of hydrogen. The cofactor ('H-cluster') of [FeFe]-hydrogenases consists of a [4Fe-4S] cluster bridged to a unique [2Fe] subcluster whose biosynthesis in vivo requires hydrogenase-specific maturases. Here we show that a chemical mimic of the [2Fe] subcluster can reconstitute apo-hydrogenase to full activity, independent of helper proteins. The assembled H-cluster is virtually indistinguishable from the native cofactor. This procedure will be a powerful tool for developing new artificial H₂-producing catalysts.

SUBMITTER: Esselborn J 

PROVIDER: S-EPMC3795299 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature