Intrapersonal and populational heterogeneity of the chemokine RANTES.

ABSTRACT:

Background

Current immunoassays for the chemokine RANTES (regulated on activation, normal T-cell expressed and secreted) are not tailored for specific isoforms that exist endogenously, despite the fact that variants with modified activity are known to exist. This is surprising in view of this protein's ubiquitous increased presence in many diseases and that the 2 established isoforms are truncated by enzymes also correlated to disease. An in-depth population survey of RANTES heterogeneity in the context of multiple diseases via a mass spectrometric immunoassay (MSIA) may resolve this issue.

Methods

We developed an MSIA for RANTES and endogenous variants apparent in human plasma. Samples from multiple cohorts of individuals (type 2 diabetes, congestive heart failure, history

SUBMITTER: Oran PE 

PROVIDER: S-EPMC3795435 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature