Determination of reliable reference genes for multi-generational gene expression analysis on C. elegans exposed to abused drug nicotine.

ABSTRACT:

Rational

More research has recently been focused on multigenerational toxicogenomics impacts. Such studies rely on behavioral as well as genetic and epigenetic analyses using various biotechniques. Of these technologies, quantitative reverse transcriptase PCR is considered as a mature discovery and validation tool. Nevertheless, the interpretation of the resulting gene expression necessitates the establishment of reliable internal controls for normalization. No study has been performed to identify reliable reference genes in multigenerational settings.

Objectives

The primary aim was to evaluate the stability of 16 reference gene candidates in Caenorhabditis elegans exposed to nicotine and their two subsequent generations for determining the most reliable reference genes for

SUBMITTER: Taki FA 

PROVIDER: S-EPMC3795882 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature