Application of time-resolved fluorescence for direct and continuous probing of release from polymeric delivery vehicles.

ABSTRACT: Though accurately evaluating the kinetics of release is critical for validating newly designed therapeutic carriers for in vivo applications, few methods yet exist for release measurement in real time and without the need for any sample preparation. Many of the current approaches (e.g. chromatographic methods, absorption spectroscopy, or NMR spectroscopy) rely on isolation of the released material from the loaded vehicles, which require additional sample purification and can lead to loss of accuracy when probing fast kinetics of release. In this study we describe the use of time-resolved fluorescence for in situ monitoring of small molecule release kinetics from biodegradable polymeric drug delivery systems. This method relies on the observation that fluorescent reporters being released fr

SUBMITTER: Viger ML 

PROVIDER: S-EPMC3795917 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature