Systemic alteration of cell-surface and secreted glycoprotein expression in malignant breast cancer cell lines.

ABSTRACT: Breast cancer cell lines express fewer transmembrane and secreted glycoproteins than nonmalignant ones. The objective of these experiments was to characterize the changes in the expression of several hundred glycoproteins quantitatively. Secreted and cell-surface glycoproteins were isolated using a glycoprotein capture protocol and then identified by tandem mass spectrometry. Glycoproteins expressed by a group of cell lines originating from malignant tumors of the breast were compared with those expressed by a nonmalignant set. The average number of spectral counts (proportional to relative protein abundance) and the total number of glycopeptides in the malignant samples were reduced to about two-thirds of the level in the nonmalignant samples. Most glycoproteins were expressed at a differ

SUBMITTER: Timpe LC 

PROVIDER: S-EPMC3796375 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature