Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures.

ABSTRACT: Surveillance of bloodstream infections (BSI) is a high priority within the hospital setting. Broth-based blood cultures are the current gold standard for detecting BSI, however they can require lengthy incubation periods prior to detection of positive samples. We set out to demonstrate the feasibility of using enzymatic template generation and amplification (ETGA)-mediated measurement of DNA polymerase activity to detect microbes from clinical blood cultures. In addition to routine-collected hospital blood cultures, one parallel aerobic blood culture was collected and immediately refrigerated until being transported for ETGA analysis. After refrigeration holding and transport, parallel-collected cultures were placed into a BACTEC incubator and ETGA time-course analysis was performed. Of th

SUBMITTER: Zweitzig DR 

PROVIDER: S-EPMC3796490 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature