Nonlinear cross-bridge elasticity and post-power-stroke events in fast skeletal muscle actomyosin.

ABSTRACT: Generation of force and movement by actomyosin cross-bridges is the molecular basis of muscle contraction, but generally accepted ideas about cross-bridge properties have recently been questioned. Of the utmost significance, evidence for nonlinear cross-bridge elasticity has been presented. We here investigate how this and other newly discovered or postulated phenomena would modify cross-bridge operation, with focus on post-power-stroke events. First, as an experimental basis, we present evidence for a hyperbolic [MgATP]-velocity relationship of heavy-meromyosin-propelled actin filaments in the in vitro motility assay using fast rabbit skeletal muscle myosin (28-29°C). As the hyperbolic [MgATP]-velocity relationship was not consistent with interhead cooperativity, we developed a cross-brid

SUBMITTER: Persson M 

PROVIDER: S-EPMC3797597 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature