A high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials.

ABSTRACT:

Objectives

Few model systems are amenable to developing multi-species biofilms in parallel under environmentally germane conditions. This is a problem when evaluating the potential real-world effectiveness of antimicrobials in the laboratory. One such antimicrobial is cetylpyridinium chloride (CPC), which is used in numerous over-the-counter oral healthcare products. The aim of this work was to develop a high-throughput microfluidic system that is combined with a confocal laser scanning microscope (CLSM) to quantitatively evaluate the effectiveness of CPC against oral multi-species biofilms grown in human saliva.

Methods

Twenty-four-channel BioFlux microfluidic plates were inoculated with pooled human saliva and fed filter-sterilized saliva for 20 h at 37°C. The bacterial di

SUBMITTER: Nance WC 

PROVIDER: S-EPMC3797639 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature